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hl1 cell viability  (Beyotime)


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    Beyotime hl1 cell viability
    Effect of PINCH1 on the function of <t>HL1</t> cells. (A) Successful PINCH1 shRNA was confirmed by western blot analysis. (B) Percentage of GFP + cells in PINCH1 shRNA/PINCH1 OE cells following transfection. Untreated cells were used as a negative control. (C) The expression levels of PINCH1 mRNA in control, PINCH1 shRNA, and PINCH1 shRNA/PINCH1 OE cells were determined by qPCR. (D) HL1 cell viability (magnification, x100) and (E) migration ability were decreased following PINCH1 shRNA (magnification, x100). (F) HL1 cell apoptosis rate was significantly increased following PINCH1 shRNA. ** P<0.05 vs. shRNA NC; ## P<0.05 vs. PINCH1 SHRNA/PINCH1 OE group. PINCH1, particularly interesting new cysteine histidine rich 1; PINCH1 shRNA, PINCH1 knockdown; PINCH1 OE, PINCH1 overexpression; PINCH1 SHRNA/PINCH1 OE, PINCH1 overexpression (PINCH1 OE) in PINCH1 shRNA HL1 cells.
    Hl1 Cell Viability, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 21913 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hl1+cell+viability/pmc08649883-34-2-19?v=Beyotime
    Average 99 stars, based on 21913 article reviews
    hl1 cell viability - by Bioz Stars, 2026-08
    99/100 stars

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    1) Product Images from "PINCH1 knockout aggravates myocardial infarction in mice via mediating the NF-κB signaling pathway"

    Article Title: PINCH1 knockout aggravates myocardial infarction in mice via mediating the NF-κB signaling pathway

    Journal: Experimental and Therapeutic Medicine

    doi: 10.3892/etm.2021.10984

    Effect of PINCH1 on the function of HL1 cells. (A) Successful PINCH1 shRNA was confirmed by western blot analysis. (B) Percentage of GFP + cells in PINCH1 shRNA/PINCH1 OE cells following transfection. Untreated cells were used as a negative control. (C) The expression levels of PINCH1 mRNA in control, PINCH1 shRNA, and PINCH1 shRNA/PINCH1 OE cells were determined by qPCR. (D) HL1 cell viability (magnification, x100) and (E) migration ability were decreased following PINCH1 shRNA (magnification, x100). (F) HL1 cell apoptosis rate was significantly increased following PINCH1 shRNA. ** P<0.05 vs. shRNA NC; ## P<0.05 vs. PINCH1 SHRNA/PINCH1 OE group. PINCH1, particularly interesting new cysteine histidine rich 1; PINCH1 shRNA, PINCH1 knockdown; PINCH1 OE, PINCH1 overexpression; PINCH1 SHRNA/PINCH1 OE, PINCH1 overexpression (PINCH1 OE) in PINCH1 shRNA HL1 cells.
    Figure Legend Snippet: Effect of PINCH1 on the function of HL1 cells. (A) Successful PINCH1 shRNA was confirmed by western blot analysis. (B) Percentage of GFP + cells in PINCH1 shRNA/PINCH1 OE cells following transfection. Untreated cells were used as a negative control. (C) The expression levels of PINCH1 mRNA in control, PINCH1 shRNA, and PINCH1 shRNA/PINCH1 OE cells were determined by qPCR. (D) HL1 cell viability (magnification, x100) and (E) migration ability were decreased following PINCH1 shRNA (magnification, x100). (F) HL1 cell apoptosis rate was significantly increased following PINCH1 shRNA. ** P<0.05 vs. shRNA NC; ## P<0.05 vs. PINCH1 SHRNA/PINCH1 OE group. PINCH1, particularly interesting new cysteine histidine rich 1; PINCH1 shRNA, PINCH1 knockdown; PINCH1 OE, PINCH1 overexpression; PINCH1 SHRNA/PINCH1 OE, PINCH1 overexpression (PINCH1 OE) in PINCH1 shRNA HL1 cells.

    Techniques Used: shRNA, Western Blot, Transfection, Negative Control, Expressing, Control, Migration, Knockdown, Over Expression

    HL1 cell apoptosis is associated with the NF-κB signaling pathway. Expression levels of NF-κB signaling pathway-related proteins were (A) determined by western blotting and (B) semi-quantified. NF-κB, MYD88, TNF-α and caspase-3 expression levels were significantly increased, whereas MMP-2 and MMP-9 expression levels were notably decreased in the shRNA PINCH1 group. ** P<0.01 vs. shRNA NC group. MYD88, myeloid differentiation factor 88; PINCH1, particularly interesting new cysteine histidine rich 1; WT, wild-type; shRNA, short hairpin RNA.
    Figure Legend Snippet: HL1 cell apoptosis is associated with the NF-κB signaling pathway. Expression levels of NF-κB signaling pathway-related proteins were (A) determined by western blotting and (B) semi-quantified. NF-κB, MYD88, TNF-α and caspase-3 expression levels were significantly increased, whereas MMP-2 and MMP-9 expression levels were notably decreased in the shRNA PINCH1 group. ** P<0.01 vs. shRNA NC group. MYD88, myeloid differentiation factor 88; PINCH1, particularly interesting new cysteine histidine rich 1; WT, wild-type; shRNA, short hairpin RNA.

    Techniques Used: Expressing, Western Blot, shRNA



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    hl1 cell viability  (Beyotime)
    99
    Beyotime hl1 cell viability
    Effect of PINCH1 on the function of <t>HL1</t> cells. (A) Successful PINCH1 shRNA was confirmed by western blot analysis. (B) Percentage of GFP + cells in PINCH1 shRNA/PINCH1 OE cells following transfection. Untreated cells were used as a negative control. (C) The expression levels of PINCH1 mRNA in control, PINCH1 shRNA, and PINCH1 shRNA/PINCH1 OE cells were determined by qPCR. (D) HL1 cell viability (magnification, x100) and (E) migration ability were decreased following PINCH1 shRNA (magnification, x100). (F) HL1 cell apoptosis rate was significantly increased following PINCH1 shRNA. ** P<0.05 vs. shRNA NC; ## P<0.05 vs. PINCH1 SHRNA/PINCH1 OE group. PINCH1, particularly interesting new cysteine histidine rich 1; PINCH1 shRNA, PINCH1 knockdown; PINCH1 OE, PINCH1 overexpression; PINCH1 SHRNA/PINCH1 OE, PINCH1 overexpression (PINCH1 OE) in PINCH1 shRNA HL1 cells.
    Hl1 Cell Viability, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hl1+cell+viability/pmc08649883-34-2-19?v=Beyotime
    Average 99 stars, based on 1 article reviews
    hl1 cell viability - by Bioz Stars, 2026-08
    99/100 stars
    		  Buy from Supplier

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    Effect of PINCH1 on the function of HL1 cells. (A) Successful PINCH1 shRNA was confirmed by western blot analysis. (B) Percentage of GFP + cells in PINCH1 shRNA/PINCH1 OE cells following transfection. Untreated cells were used as a negative control. (C) The expression levels of PINCH1 mRNA in control, PINCH1 shRNA, and PINCH1 shRNA/PINCH1 OE cells were determined by qPCR. (D) HL1 cell viability (magnification, x100) and (E) migration ability were decreased following PINCH1 shRNA (magnification, x100). (F) HL1 cell apoptosis rate was significantly increased following PINCH1 shRNA. ** P<0.05 vs. shRNA NC; ## P<0.05 vs. PINCH1 SHRNA/PINCH1 OE group. PINCH1, particularly interesting new cysteine histidine rich 1; PINCH1 shRNA, PINCH1 knockdown; PINCH1 OE, PINCH1 overexpression; PINCH1 SHRNA/PINCH1 OE, PINCH1 overexpression (PINCH1 OE) in PINCH1 shRNA HL1 cells.

    Journal: Experimental and Therapeutic Medicine

    Article Title: PINCH1 knockout aggravates myocardial infarction in mice via mediating the NF-κB signaling pathway

    doi: 10.3892/etm.2021.10984

    Figure Lengend Snippet: Effect of PINCH1 on the function of HL1 cells. (A) Successful PINCH1 shRNA was confirmed by western blot analysis. (B) Percentage of GFP + cells in PINCH1 shRNA/PINCH1 OE cells following transfection. Untreated cells were used as a negative control. (C) The expression levels of PINCH1 mRNA in control, PINCH1 shRNA, and PINCH1 shRNA/PINCH1 OE cells were determined by qPCR. (D) HL1 cell viability (magnification, x100) and (E) migration ability were decreased following PINCH1 shRNA (magnification, x100). (F) HL1 cell apoptosis rate was significantly increased following PINCH1 shRNA. ** P<0.05 vs. shRNA NC; ## P<0.05 vs. PINCH1 SHRNA/PINCH1 OE group. PINCH1, particularly interesting new cysteine histidine rich 1; PINCH1 shRNA, PINCH1 knockdown; PINCH1 OE, PINCH1 overexpression; PINCH1 SHRNA/PINCH1 OE, PINCH1 overexpression (PINCH1 OE) in PINCH1 shRNA HL1 cells.

    Article Snippet: To determine HL1 cell viability, a Cell Counting Kit 8 (CCK-8) assay was used according to the manufacturer's instructions (Beyotime Institute of Biotechnology).

    Techniques: shRNA, Western Blot, Transfection, Negative Control, Expressing, Control, Migration, Knockdown, Over Expression

    HL1 cell apoptosis is associated with the NF-κB signaling pathway. Expression levels of NF-κB signaling pathway-related proteins were (A) determined by western blotting and (B) semi-quantified. NF-κB, MYD88, TNF-α and caspase-3 expression levels were significantly increased, whereas MMP-2 and MMP-9 expression levels were notably decreased in the shRNA PINCH1 group. ** P<0.01 vs. shRNA NC group. MYD88, myeloid differentiation factor 88; PINCH1, particularly interesting new cysteine histidine rich 1; WT, wild-type; shRNA, short hairpin RNA.

    Journal: Experimental and Therapeutic Medicine

    Article Title: PINCH1 knockout aggravates myocardial infarction in mice via mediating the NF-κB signaling pathway

    doi: 10.3892/etm.2021.10984

    Figure Lengend Snippet: HL1 cell apoptosis is associated with the NF-κB signaling pathway. Expression levels of NF-κB signaling pathway-related proteins were (A) determined by western blotting and (B) semi-quantified. NF-κB, MYD88, TNF-α and caspase-3 expression levels were significantly increased, whereas MMP-2 and MMP-9 expression levels were notably decreased in the shRNA PINCH1 group. ** P<0.01 vs. shRNA NC group. MYD88, myeloid differentiation factor 88; PINCH1, particularly interesting new cysteine histidine rich 1; WT, wild-type; shRNA, short hairpin RNA.

    Article Snippet: To determine HL1 cell viability, a Cell Counting Kit 8 (CCK-8) assay was used according to the manufacturer's instructions (Beyotime Institute of Biotechnology).

    Techniques: Expressing, Western Blot, shRNA